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Journal / Neuroscience / Optogenetic Aktivering av sebrafisk…
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JoVE Journal Neuroscience
Optogenetic Aktivering av sebrafisk somatosensoriske Neurons bruker Chef-tdTomato
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Optogenetic Aktivering av sebrafisk somatosensoriske Neurons bruker Chef-tdTomato

Article DOI: 10.3791/50184-v • 07:25 min • January 31st, 2013
January 31st, 2013 •
1, 1
1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles

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Optogenetic teknikker har gjort det mulig å studere bidraget av spesifikke nerveceller til atferd. Vi beskriver en metode i larve sebrafisk for aktivering enkelt somatosensoriske nevroner uttrykker en channelrhodopsin variant (kokk) med en diode-pumpet solid state (DPSS) laser og registrering av elicited atferd med et høyhastighets videokamera.

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Nevrovitenskap utviklingsbiologi molekylær biologi cellebiologi biokjemi bioteknologi anatomi fysiologi sebrafisk Behavior Animal Touch optogenetics channelrhodopsin kokk sensorisk nevron Rohon-Beard, Nevroner mikroinjeksjon konfokalmikroskopi høyhastighets video dyremodell
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