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Naïve Adult Stem Cells isolamento da colture primarie di fibroblasti umani
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Naïve Adult Stem Cells isolamento da colture primarie di fibroblasti umani

Article DOI: 10.3791/50185-v • 07:39 min • May 3rd, 2013
May 3rd, 2013 •
1, 1, 2, 1
1Department of Dermatology and Institute for Medical Engineering, Technische Universität München, 2Department of Dermatology and Allergology, Technische Universität München

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Riportiamo un metodo per isolare precursori pelle-derivato multipotenti (SKP) cellule naïve da colture primarie di fibroblasti umani. Abbiamo dimostrato che queste SKPs derivati ​​da colture di fibroblasti Share proprietà delle cellule staminali a quelle derivate direttamente da biopsie cutanee umane. Queste cellule esprimono il marcatore cresta neurale, nestin, oltre ai marcatori multipotenti quali OCT4 e Nanog.

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Stem Cell Biology Numero 75 Biologia Cellulare Biologia Molecolare Anatomia Fisiologia Ingegneria Biomedica Medicina Dermatologia Celle Colta cellule staminali biologia (generale) della pelle e delle malattie del tessuto connettivo fenomeni biologici cellule staminali adulte pelle derivato cellule precursori fibroblasti cultura sfera precursori della pelle-derivati SKP PCR qPCR immunocitochimica l'isolamento la coltura cellulare
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