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Naive voksne stamceller Isolering fra primære humane fibroblastkulturer
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Naive voksne stamceller Isolering fra primære humane fibroblastkulturer

Article DOI: 10.3791/50185-v • 07:39 min • May 3rd, 2013
May 3rd, 2013 •
1, 1, 2, 1
1Department of Dermatology and Institute for Medical Engineering, Technische Universität München, 2Department of Dermatology and Allergology, Technische Universität München

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Vi rapporterer en metode til at isolere naive multipotente hud-afledt precursor (SKP) celler fra primære humane fibroblastkulturer. Vi viser, at disse SKPs stammer fra fibroblastkulturer deler lignende stamceller egenskaber til dem stammer direkte fra humane hudbiopsier. Disse celler udtrykker neuralkam markering, nestin, ud over de multipotente markører, såsom OCT4 og Nanog.

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Stem Cell Biology cellebiologi molekylærbiologi anatomi fysiologi Biomedical Engineering Medicine dermatologi Cells Cultured Stamceller biologi (generelt) hud og bindevævssygdomme biologiske fænomener voksne stamceller hud afledt precursor celler fibroblaster sfære kultur hud-afledte prækursorer SKP PCR qPCR immuncytokemi isolation cellekultur
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