A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Oncology,Georgetown University, 2Lombardi Comprehensive Cancer Center,Georgetown University, 3Stem Cell Dynamics,Helmholtz Zentrum München – German Research Center for Environmental Health, 4Department of Medicine,Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science,Dankook University
Chapters
- 00:05Title
- 01:48Generation of Primary Mammary Epithelial Cell Cultures
- 03:39Live-cell Imaging
- 05:56Assessing Cellular Event from Time-lapse Images
- 07:50Making Single Cell Fate Maps with Timm’s Tracking Tool (TTT)
- 09:59Representative Results: Colony Formation in BRCA1 Disrupted Cells
- 11:12Conclusion
Time-lapse bildebehandling brukes til å vurdere oppførselen primære preneoplastic mammary epitelceller avledet fra genmanipulerte mus modeller av risikoen for brystkreft for å avgjøre om det er sammenhenger mellom spesifikke atferdsmessige parametere og distinkte genetiske lesjoner.
