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Giant liposomfremstilling for billedbehandling og Patch-Clamp Elektrofysiologi
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Giant liposomfremstilling for billedbehandling og Patch-Clamp Elektrofysiologi

Article DOI: 10.3791/50227-v • 09:03 min • June 21st, 2013
June 21st, 2013 •
1, 1
1Department of Physiology and Biophysics, University of Washington

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Færdigtilberedning funktionelle membranproteiner i kæmpeliposomer af definerede sammensætning er en kraftfuld metode, når det kombineres med patch-clamp elektrofysiologi. Dog kan konventionelle kæmpe liposomproduktion være uforeneligt med protein stabilitet. Vi beskriver protokoller til at lave kæmpeliposomer fra rene lipider eller små liposomer indeholdende ionkanaler.

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Fysiologi Biofysik molekylærbiologi biokemi genetik cellebiologi Proteins Membraner Kunstige lipiddobbeltlagene liposomer fosfolipider biokemi lipider Giant unilamelvesikler liposom elektrofysiologi electroformation rekonstitution patch clamp
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