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Protocolo melhorado para microdissecção a laser de ilhotas pancreáticas humanas a partir de espécimes cirúrgicos
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Protocolo melhorado para microdissecção a laser de ilhotas pancreáticas humanas a partir de espécimes cirúrgicos

Article DOI: 10.3791/50231-v • 09:44 min • January 6th, 2013
January 6th, 2013 •
1,2, 3, 1,2, 1, 2, 1,2, 2, 4, 5, 6, 7, 8, 9, 2, 10, 3, 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

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Microdissecção a laser é uma técnica que permite a recuperação de células selecionadas a partir de pequenas quantidades de parênquima. Aqui descrevemos um protocolo de aquisição ilhotas pancreáticas humanas a partir de espécimes cirúrgicos para serem utilizados para estudos de transcriptomic. Nosso protocolo melhora a autofluorescência intrínseca das células beta humanas, facilitando assim a sua coleção.

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Medicina Edição 71 Fisiologia Anatomia Bioquímica Biologia Celular Biologia Molecular Imunologia Cirurgia Diabetes Mellitus Tipo 2 microdissecção a laser a dissecção as células beta humanas autofluorescência intrínseca pâncreas ressecção parcial Diabetes tipo 2 os estudos transcriptomic RNA análise ilhota
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