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RNA Secondary Structure Prediction Mit High-Throughput-SHAPE
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RNA Secondary Structure Prediction Mit High-Throughput-SHAPE

Article DOI: 10.3791/50243-v • 13:42 min • May 31st, 2013
May 31st, 2013 •
*1, *1, 1, 1, 1
1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research
* These authors contributed equally

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Hochdurchsatz-selektiven 2-Hydroxyl-Acylierung durch Primer-Extension (FORM) analysiert verwendet einen neuartigen chemischen Tastertechnologie, reverse Transkription, Kapillarelektrophorese und Sekundärstrukturvorhersage Software, um die Strukturen der RNAs, die von mehreren hundert bis zu mehreren tausend bestimmen Nukleotide an Einzel-Nukleotid-Auflösung.

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Genetik Molekularbiologie Biochemie Virologie Krebs Biologie Medizin Genomics Nukleinsäure-Sonden RNA-Sonden RNA Hochdurchsatz-SHAPE Kapillarelektrophorese RNA-Struktur RNA Sondieren RNA-Faltung sekundäre Struktur DNA Nukleinsäuren Elektropherogramm Synthese Transkription hoher Durchsatz Sequenzierung
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