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JoVE Journal Bioengineering
Generering af Aligned Functional myocardievæv via microcontact printing
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Generering af Aligned Functional myocardievæv via microcontact printing

Article DOI: 10.3791/50288-v • 11:09 min • March 19th, 2013
March 19th, 2013 •
1, 1,2
1Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, 2Harvard Stem Cell Institute

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Dannelsen af ​​linie myocardievæv er en central forudsætning for at tilpasse de seneste fremskridt inden for stamcelle biologi til klinisk nyttige formål. Heri beskrives en microcontact printing fremgangsmåde til præcis regulering af celle-form og funktion. Brug højtoprensede populationer af embryonale stamceller afledt hjerte progenitorer, så genererer vi anisotropisk funktionel myokardievæv.

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Stem Cell Biology Bioengineering Biomedical Engineering medicin molekylærbiologi cellebiologi anatomi fysiologi Tissue Engineering Cardiology Cell Biology embryonale stamceller økonomiske og sociale råd Micropatterning microcontact printing Cellejustering Heart progenitorer, transgene mus mus embryonale stamceller stamceller myocardievæv PDMS FACS flowcytometri dyremodel
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