Abstract
The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.
Protocol
The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect. The volume has been corrected from:
The spermidine is unstable in aqueous solution. We make 70μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.
to:
The spermidine is unstable in aqueous solution. We make 16μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.
Disclosures
No conflicts of interest declared.
