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Langfristige Silencing von Intersectin-1s in Mauslungen von wiederkehrenden Lieferung eines spezifischen siRNA durch kationische Liposomen. Auswertung der Knockdown Effects Electron Microscopy
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Langfristige Silencing von Intersectin-1s in Mauslungen von wiederkehrenden Lieferung eines spezifischen siRNA durch kationische Liposomen. Auswertung der Knockdown Effects Electron Microscopy

Article DOI: 10.3791/50316-v • 15:55 min • June 21st, 2013
June 21st, 2013 •
*1, *1,2, 1,2
1Department of Pharmacology, Rush University, 2Pulmonary and Critical Care Department, Rush University
* These authors contributed equally

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Wiederholte retroorbitalen Injektionen kationische liposomes/siRNA/ITSN-1s Komplexe in Mäusen, alle 72 Stunden für 24 Tage, effizient die siRNA-Duplex zum Mauslunge Mikrovaskulatur Reduzierung ITSN-1s mRNA und Protein-Expression von 75%. Diese Technik ist in hohem Maße reproduzierbar in einem Tiermodell, hat keine Nebenwirkungen und Todesfälle verhindert.

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Bioengineering Biomedical Engineering Biochemie Genetik Molekularbiologie Zellbiologie Anatomie Physiologie Medizin Immunologie Pharmakologie Tiermodelle Herz-Kreislauf-Krankheiten Intersectin-1s siRNA Liposomen retro-orbitale Injektion akute und chronische ITSN-1s Knockdown transgene Mäuse Liposom endothelialen Zellen Gewebe- Lungen- Perfusion Elektronenmikroskopie Tiermodell
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