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Journal / Biology / GST-Hans oprensning: En to-trins…
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GST-Hans oprensning
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GST-Hans oprensning: En to-trins Affinitetsrensning protokollen Efterlevelse fuld længde oprensede proteiner

Article DOI: 10.3791/50320-v • 11:22 min • October 29th, 2013
October 29th, 2013 •
*1, *1, *1, 1, 1, 1
1Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec
* These authors contributed equally

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I den foreliggende protokol, viser vi en yderst effektiv og omkostningseffektiv små proteinoprensning metode, som tillader oprensning af rekombinante proteiner ved entydigt at kombinere en spaltelig GST-tag og en lille His-mærke.

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Biokemi genetik molekylærbiologi Proteiner Proteomics rekombinant protein affinitetsrensning Glutathione Sepharose Tag Talon metal affinitetsharpiks
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