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GST-His-Reinigung
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GST-His-Reinigung: Ein Zwei-Schritt-Affinitätsreinigung Protokoll Nachgeben in voller Länge Gereinigte Proteine

Article DOI: 10.3791/50320-v • 11:22 min • October 29th, 2013
October 29th, 2013 •
*1, *1, *1, 1, 1, 1
1Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec
* These authors contributed equally

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In dem vorliegenden Protokoll, zeigen wir eine hocheffiziente und kostengünstige kleine Proteinreinigungsverfahren, die die Reinigung von rekombinanten Proteinen ermöglicht dank einer einzigartigen Kombination eine spaltbare GST-Tag und einen kleinen His-Tag.

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Biochemie Genetik Molekularbiologie Proteine Proteomics rekombinantes Protein Affinitätsreinigung Glutathion-Sepharose Tag Talon Metallaffinitätsharz
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