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Journal / Biology / GST-Su purificación: A dos pasos Affinity…
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GST-Su purificación
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GST-Su purificación: A dos pasos Affinity Protocolo Purificación Cediendo largos proteínas purificadas

Article DOI: 10.3791/50320-v • 11:22 min • October 29th, 2013
October 29th, 2013 •
*1, *1, *1, 1, 1, 1
1Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec
* These authors contributed equally

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En el presente protocolo, se demuestra un método de purificación de proteínas a pequeña escala altamente eficiente y rentable, que permite la purificación de proteínas recombinantes, combinando de forma única una etiqueta de GST escindible y una pequeña cola de His.

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Bioquímica Genética Biología Molecular proteínas proteómica la proteína recombinante purificación por afinidad glutatión Sepharose Tag resina de afinidad de metal Talon
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