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Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

Article DOI: 10.3791/50339-v • 09:17 min • April 24th, 2013
April 24th, 2013 •
1, 1
1Department of Biomolecular Genetics, University of Rochester Medical Center

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Here we describe two assays that have been established to study age-dependent neurodegeneration of dopaminergic (DA) neurons in Drosophila: the climbing/startle-induced negative geotaxis assay which allows to study the functional effects of DA neurons degeneration and the tyrosine hydroxylase immunostaining which is used to identify and count DA neurons in whole brain mounts.

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Neurodegenerative Phenotypes Drosophila Melanogaster Dopaminergic Neurons Climbing Assays Whole Brain Immunostaining Aging Pathological Degenerative Processes Nervous System Fly Model Organism Genetic Tractability Short Life Span Complex Behaviors Disease-linked Genes Neurodegenerative Diseases Parkinson's Disease Alzheimer's Disease Loss Of DA Neurons Environmental Toxins Mutations In Coding Or Promoter Regions PD-associated Alleles α-synuclein Gene Expression
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