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Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining
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Neuroscience
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JoVE Journal Neuroscience
Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

DOI:
10.3791/50339-v

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09:17 min

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April 24, 2013

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,
1Department of Biomolecular Genetics,University of Rochester Medical Center

Chapters

  • 00:05Title
  • 01:10Startle-induced Negative Geotaxis Assay
  • 03:14Negative Geotaxis Assay Data Analysis
  • 04:07Dissecting Adult Drosophila Brains for Whole Mounts
  • 05:14Tyrosine Hydroxylase Immunofluorescence of Whole Mount Brains
  • 08:10Investigating the Stress-protective Nrf2 Pathway in a Drosophila Model of PD
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe two assays that have been established to study age-dependent neurodegeneration of dopaminergic (DA) neurons in Drosophila: the climbing/startle-induced negative geotaxis assay which allows to study the functional effects of DA neurons degeneration and the tyrosine hydroxylase immunostaining which is used to identify and count DA neurons in whole brain mounts.

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Keywords: DrosophilaDopaminergic NeuronsNeurodegenerative PhenotypesClimbing AssayWhole Brain ImmunostainingParkinson’s DiseaseAlpha-synucleinTyrosine Hydroxylase

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