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Montering af nukleosomal Arrays fra rekombinant Core Histoner og nukleosom Positioning DNA
 
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Montering af nukleosomal Arrays fra rekombinant Core Histoner og nukleosom Positioning DNA

Article DOI: 10.3791/50354-v 10:40 min September 10th, 2013
September 10th, 2013

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Summary

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Click here for the English version.

En metode præsenteres for rekonstituering af model nukleosomal arrays fra rekombinante kerne histoner og tandemgentaget DNA nukleosom positionering. Vi beskriver også, hvordan sedimentationshastighed eksperimenter i den analytiske ultracentrifuge og atomic force mikroskopi (AFM) anvendes til at overvåge omfanget af nukleosomal matrix mætning efter rekonstitution.

Tags

Cellebiologi Kromosom Konstruktioner kromatin nukleosomer Histoner Microscopy Atomic Force (AFM) biokemi kromatin nukleosom nukleosomal Array histon Analytical Ultracentifugering Sedimentation Velocity
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