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Journal / Biology / Montering av Nucleosomal Arrays fra Rekombinant…
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Montering av Nucleosomal Arrays fra Rekombinant kjerne Histoner og nukleosom Positioning DNA
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Montering av Nucleosomal Arrays fra Rekombinant kjerne Histoner og nukleosom Positioning DNA

Article DOI: 10.3791/50354-v • 10:40 min • September 10th, 2013
September 10th, 2013 •
1, 1, 1, 1, 1, 1
1Biochemistry and Molecular Biology, Colorado State University

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En metode er presentert for tilberedning av modell nucleosomal arrays fra rekombinante kjerne histoner og tandem gjentas nukleosom posisjonering DNA. Vi beskriver også hvordan sedimente velocity eksperimenter i den analytiske ultrasentrifuge, og atomic force mikroskopi (AFM) er brukt til å overvåke omfanget av nucleosomal rekke metning etter tilberedning.

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Cellular Biology Kromosom Structures Kromatin Nucleosomes Histoner Mikroskopi Atomic Force (AFM) biokjemi Kromatin nukleosom Nucleosomal Array Histone Analytical ultracentrifugation Sedimente Velocity
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