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Visualisering og analyse af mRNA molekyler under anvendelse Fluorescens<em> In Situ</em> Hybridisering i<em> Saccharomyces cerevisiae</em
JoVE Journal
Biology
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JoVE Journal Biology
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualisering og analyse af mRNA molekyler under anvendelse Fluorescens<em> In Situ</em> Hybridisering i<em> Saccharomyces cerevisiae</em

DOI:
10.3791/50382-v

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07:00 min

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June 14, 2013

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, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 01:11Coverslip Preparation
  • 01:51Fixation
  • 03:08Hybridization
  • 04:53Imaging of Cells with Olympus IX-81 Inverted Microscope
  • 05:23Results: Quantitative mRNA Analysis by FISH
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en eksperimentel procedure for udførelse af fluorescens<em> In situ</em> Hybridisering (FISH) til optælling mRNA i enkelte celler på single-molekyle opløsning.

Tags

Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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