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JoVE Journal Biology
Udarbejdelse af pancreas acinarceller til brug ved Calcium Imaging, celleskade Målinger og adenovirusinfektion
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Udarbejdelse af pancreas acinarceller til brug ved Calcium Imaging, celleskade Målinger og adenovirusinfektion

Article DOI: 10.3791/50391-v • 10:39 min • July 5th, 2013
July 5th, 2013 •
1, 1, 1, 1, 2, 1
1Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC, 2Department of Surgery, Tufts University Medical Center

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Vi beskriver en reproducerbar fremgangsmåde til fremstilling af muse pankreatiske acinarceller fra en mus for at undersøge acinar celle calcium signaler og cellulær skade med fysiologisk og patologisk relevante stimuli. En fremgangsmåde til adenoviral infektion af disse celler er også tilvejebragt.

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Cancer Biology cellebiologi molekylærbiologi medicin biokemi Biomedical Engineering acinarceller pancreatitis Transfection Microscopy Confocal Calcium signalering Pancreas acinarceller pancreatitis Calcium signalering cytotoksicitet LDH Lækage celle-skader imaging
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