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Journal / Immunology and Infection / Isolation of Precursor B-cell Subsets from…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Article DOI: 10.3791/50402-v • 14:06 min • April 16th, 2013
April 16th, 2013 •
1, 1, 2, 1, 1
1Department of Pathology and Anatomical Sciences, University of Missouri-Columbia, 2Laboratory for Infectious Disease Research, University of Missouri-Columbia

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Summary
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Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.

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Isolation Precursor B-cell Subsets Umbilical Cord Blood Hematopoietic Progenitor Cells Differentiation Stages Protocol Genes Expression Epigenetic Modifications Tissue Specificity Cell Types Discrimination Genome Alterations Epigenome Alterations Disease Development Adaptation To Cell Types Mononuclear Cells Separation Density Centrifugation B-cell Enrichment Biotin Conjugated Antibodies Non B-cells Removal Fluorescent Labeling Cell Surface Protein Antibodies Sorting Of Cells Nucleic Acid Assays
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