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Journal / Immunology and Infection / En enkel og effektiv metode til at påvise Nuclear …
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Immunology and Infection

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JoVE Journal Immunology and Infection
En enkel og effektiv metode til at påvise Nuclear Factor Aktivering i humane neutrofiler ved flowcytometri
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En enkel og effektiv metode til at påvise Nuclear Factor Aktivering i humane neutrofiler ved flowcytometri

Article DOI: 10.3791/50410-v • 08:26 min • April 9th, 2013
April 9th, 2013 •
1, 2, 3
1Department of Biological Sciences, University of Alberta, 2División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, 3Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México

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Neutrofiler er de mest udbredte leukocytter i blodet. Neutrofiler besidder transkriptionelt regulerede funktioner, såsom produktion af proinflammatoriske cytokiner og inhibering af apoptose. Disse funktioner kan undersøges med fremgangsmåden præsenteret her, som muliggør påvisning og kvantificering af nukleare faktorer ved flowcytometri i isolerede kerner

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Immunology Biochemistry infektion Cellular Biology molekylærbiologi medicin Neutrofiler neutrofile monocyt PMN NF-KB ERK integrin signaltransduktion inflammation flowcytometri immunolabeling nukleare faktorer cytokiner celler assay
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