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JoVE Journal Neuroscience
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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Article DOI: 10.3791/50412-v • 11:48 min • July 24th, 2013
July 24th, 2013 •
*1, *2, 1, 1
1Institute of Anatomy and Cell Biology, University of Heidelberg, 2Institut de recherches cliniques de Montreal
* These authors contributed equally

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Summary
Automatic Translation

Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

Tags

Genetic Manipulation Mouse Developing Hypothalamus In Utero Electroporation Novel Mouse Mutants Mouse Brain Cortex Thalamus Hypothalamus Transfection Neuroepithelium Electroporation Narcosis Times Third Ventricle Electrodes Mammillary Body Histological Analysis
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