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Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Article DOI: 10.3791/50425-v • 09:11 min • November 14th, 2016
November 14th, 2016 •
1,2, 1,3, 2, 2, 2, 1
1Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Institutet, 2Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, 3Department of Medical Biochemistry and Biophysics, Vascular Biology Unit, Karolinska Institutet

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Summary
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We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

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Immunohistochemical Analysis Rat Central Nervous System Peripheral Lymph Node Tissue Sections Specific Protein Isolating Fixing Paraffin-embedding Sectioning Mounting Deparaffinize Rehydrate Block Endogenous Peroxidase Antigen Retrieval Blocking Antibodies Immunolabeling Primary Antibodies Secondary Antibodies Signal Development Light Microscopy Protein Localization Pattern Insight Into Protein Localization And Expression Patterns Other Organs And Species Technician Katalin Benedek Tissue Preparation Paraformaldehyde
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