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Journal / Chemistry / Målrettede Metabolomics fra biologiske kilder…
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JoVE Journal Chemistry
Målrettede Metabolomics fra biologiske kilder Brug Ultraperformance væskekromatografi-højopløsningsmassespektrometri (UPLC-HRMS)
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Målrettede Metabolomics fra biologiske kilder Brug Ultraperformance væskekromatografi-højopløsningsmassespektrometri (UPLC-HRMS)

Article DOI: 10.3791/50433-v • 11:00 min • May 20th, 2013
May 20th, 2013 •
1, 1, 1, 1, 1
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania

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Målrettede metabolomics giver en hypotese genererer øjebliksbillede af en metabolisk profil. Denne protokol vil demonstrere ekstraktion og analyse af metabolitter fra celler, serum eller væv. En række metabolitter undersøges ved hjælp af væske-væske fase ekstraktion, Microflow ultraperformance væskekromatografi / høj opløsning massespektrometri (UPLC-HRMS) koblet til differential analyse software.

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Biokemi Kemi Molekylærbiologisk Institut cellebiologi fysiologi medicin farmakologi genetik Genomics massespektrometri MS Metabolisme Metabolomics ikke-målrettede ekstraktion lipider præcis masse væskekromatografi ultraperformance væskekromatografi UPLC højopløsningsmassespektrometri HRMS spektrometri
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