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JoVE Journal Biology
Levende Cell Imaging af Early Autophagy begivenheder
Fluorescence Imaging with One-nanometer Accuracy (FIONA)
Journal (Biology)
Fluorescence Imaging with One-nanometer Accuracy (FIONA)
Intravital Microscopy of the Microcirculation in the Mouse Cremaster Muscle for the Analysis of Peripheral Stem Cell Migration
Journal (Biology)
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Levende Cell Imaging af Early Autophagy begivenheder: Omegasomes and Beyond

Article DOI: 10.3791/50484-v • 09:00 min • July 27th, 2013
July 27th, 2013 •
1, 2, 1, 1, 1
1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University

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Time-lapse mikroskopi af fluorescens-mærkede autophagy markører muligt at overvåge den dynamiske autophagy respons med høj tidslig opløsning. Ved hjælp af konkrete autophagy og organel markører i en kombination af 3 forskellige farver, kan vi følge det bidrag af et protein til autophagosome dannelse i en robust rumlig og tidsmæssig sammenhæng.

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Cellular Biology Molekylærbiologi biokemi phosphatidylinositoler Microscopy Fluorescens Video Autophagy Cell Biology Autophagy Omegasome DFCP1 LC3 Levende billedbehandling Time-lapse mikroskopi cell billedbehandling
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