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JoVE Journal Neuroscience
Laser Capture mikrodissektion af neuroner fra differentierede humane Neuroprogenitor celler i kultur
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Laser Capture mikrodissektion af neuroner fra differentierede humane Neuroprogenitor celler i kultur

Article DOI: 10.3791/50487-v • 12:38 min • September 16th, 2013
September 16th, 2013 •
1,2, 1,2, 1,2
1Section of Endocrinology, Denver VA Medical Center, 2Department of Medicine, University of Colorado Denver School of Medicine

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Humane neuroprogenitor celler (NPCs) blev udvidet under prolifererende forhold. NPC blev differentieret i neuron-rige kulturer i nærvær af en kombination af neurotrophiner. Neuronale markører blev påvist ved immunfluorescens-farvning. At isolere en ren population af neuroner blev NPC'ere differentieres på PEN membran dias og laser capture mikrodissektion blev udført.

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Neuroscience neurobiologi cellebiologi Celler kulturperler neuroner centralnervesystemet neurodegenerative sygdomme menneskelige neuroprogenitor celler neuronal differentiering neuronale markører astrocytter laser capture mikrodissektion PEN membran dias cellekultur
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