Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Nataliya I. Skrypnyk; Raymond C. Harris; Mark P. de Caestecker

doi:10.3791/50495

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

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Medicine

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JoVE Journal Medicine

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

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09:09 min

August 9, 2013

DOI: 10.3791/50495-v

Nataliya I. Skrypnyk¹, Raymond C. Harris¹, Mark P. de Caestecker¹

¹Division of Nephrology,Vanderbilt University Medical Center

We describe models of moderate and severe ischemia-reperfusion-induced kidney injury in which the mice undergo unilateral renal pedicle clamping followed by simultaneous or delayed contralateral nephrectomy, respectively. These models consistently give rise to renal dysfunction and post-injury fibrosis, but injury severity and survival are dependent on mouse background, age and surgical equipment.

The overall goal of this procedure is to induce a consistent survivable and functionally measurable degree of ischemia. Reperfusion induced acute kidney injury in mice by performing unilateral renal pedicle clamping, followed by contralateral nephrectomy ischemia. Reperfusion injury is achieved by making a dorsal incision and exposing the left and right renal pedicle.

That includes the renal artery and vein. Next, a contralateral nephrectomy is performed after liberating the kidney from the surrounding tissue. Then the left renal pedicle is clamped using a vascular clamp and the kidney is covered with the skin.

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