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Journal / Biology / 定量检测，以研究蛋白质：DNA的相互作用，发现转录基因表达的调节，并确定新的抗肿瘤剂…
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定量检测，以研究蛋白质：DNA的相互作用，发现转录基因表达的调节，并确定新的抗肿瘤剂
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定量检测，以研究蛋白质：DNA的相互作用，发现转录基因表达的调节，并确定新的抗肿瘤剂

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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我们开发了一种定量的DNA结合，基于ELISA的测定法来测量与DNA的转录因子相互作用。高特异性的RUNX2蛋白与共识的DNA寡核苷酸识别和特异性单克隆抗体来实现。比色检测用酶偶联抗体底物反应的实时监测。

Tags

细胞生物学，78期，转录因子，维生素D，药物发现，酶联免疫吸附试验（ELISA），DNA结合，转录因子，药物筛选，抗体
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