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En kvantitativ analyse til at studere Protein
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En kvantitativ analyse til at studere Protein: DNA interaktioner, Discover transskriptionsregulatorer af genekspression, og identificere nye anti-tumor Agents

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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Vi har udviklet en kvantitativ DNA-bindende ELISA-assay til måling af transkriptionsfaktor interaktion med DNA. Høj specificitet for RUNX2 protein blev opnået med en konsensus DNA-genkendelse oligonukleotid og specifikt monoklonalt antistof. Kolorimetrisk detektion med et enzym-koblet antistof substrat Reaktionen blev overvåget i realtid.

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Cellebiologi transkriptionsfaktorer D-vitamin Drug Discovery enzymmaerket Assay (ELISA) DNA-bindende transskription faktor narkotika screening antistof
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