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JoVE Journal Biology

الأيضية وصفها من لوسين ريتش كرر تحركات 1 و 2 مع المشعة الفوسفات

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Journal (Biology)

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Understanding Cerebellar Pattern Formation

Journal (Biology)

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الأيضية وصفها من لوسين ريتش كرر تحركات 1 و 2 مع المشعة الفوسفات

Article DOI: 10.3791/50523 • 11:31 min • September 18th, 2013

September 18th, 2013 •

Jean-Marc Taymans¹, Fangye Gao¹, Veerle Baekelandt¹

¹Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven and Leuven Institute for Neuroscience and Disease (LIND)

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يسين الغنية تكرار تحركات 1 و 2 (LRRK1 وLRRK2) هي بروتينات متعددة المجالات التي ترميز كل من GTPase والمجالات كيناز والتي هي فسفرته في الخلايا. هنا، نقدم بروتوكول لتسمية LRRK1 وLRRK2 في الخلايا مع ³² P الفوسفاتية، وبالتالي توفير وسيلة لقياس مستويات الشاملة phophorylation الخلوية.

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علم الأحياء الخلوية، العدد 79، البيولوجيا عموما، والكيمياء الحيوية والهندسة الحيوية (عامة)، LRRK1، LRRK2، ووضع العلامات الأيضية، 32P الفوسفاتية، مناعي، تصوير الإشعاع الذاتي

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