Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐
 
Click here for the English version

富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐

Article DOI: 10.3791/50523-v 11:31 min September 18th, 2013
September 18th, 2013

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

富含亮氨酸的重复激酶1和2(LRRK1和LRRK2)是多结构域蛋白,其编码GTP酶都和激酶结构域和它们磷酸化的细胞中。在这里,我们提出了一个协议,以32磷正磷酸盐标记LRRK1和LRRK2的细胞,从而提供衡量其整体的细胞磷酸化水平的一种手段。

Tags

细胞生物学,79期,生物学(普通),生物化学,生物工程(总称),LRRK1,LRRK2,代谢标记,32P正磷酸盐,免疫沉淀,放射自显影
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter