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Visualization of Craniofacial Development in the sox10
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Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

Article DOI: 10.3791/50525-v • 06:35 min • September 30th, 2013
September 30th, 2013 •
1, 1, 1
1Center for Regenerative Medicine, Massachusetts General Hospital

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Visualization of experimental data has become a key element in presenting results to the scientific community. Generation of live time-lapse recording of growing embryos contributes to better presentation and understanding of complex developmental processes. This protocol is a step-by-step guide to cell labeling via photoconversion of kaede protein in zebrafish.

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Visualization Craniofacial Development Sox10: Kaede Transgenic Zebrafish Line Time-lapse Confocal Microscopy Vertebrate Palatogenesis Cranial Neural Crest (CNC) Cells Facial Prominences Craniofacial Skeleton Lineage Analysis Maxillary Elements Mandibular Elements Amniotes Sox10: Kaede Zebrafish Embryo Ethmoid Plate Time-lapse Confocal Recording Photoconvertible Reporter Protein Transgenic Zebrafish Abnormal Development Craniofacial Structure
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