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JoVE Journal Neuroscience
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Exame do Synaptic Vesicle Reciclagem Usando FM Corantes Durante Evocado, espontânea, e as atividades de Synaptic miniatura

Article DOI: 10.3791/50557-v • 08:10 min • March 31st, 2014
March 31st, 2014 •
1, 1, 1, 2, 1
1Department of Molecular Physiology & Biophysics, University of Iowa Carver College of Medicine, 2Department of Biology & Biochemistry, University of Bath

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Descreve-se o uso de corantes estiril FM imagem sináptica reciclagem vesícula nos terminais nervosos funcionais. Este protocolo pode ser aplicado não só a evocada, mas também espontânea e actividades sinápticas em miniatura. O protocolo expande a variedade de eventos sinápticos que podem ser eficazmente analisados.

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Neurociência Edição 85 terminais pré-sinápticos Synaptic vesículas Microscopia Bioensaios Sistema Nervoso endocitose exocitose imagens de fluorescência corante FM neurônio fotodegradação
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