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Journal / Neuroscience / Undersøgelse af synaptiske vesikel Genbrug Brug…
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JoVE Journal Neuroscience
Undersøgelse af synaptiske vesikel Genbrug Brug af FM Farvestoffer Under Evoked, spontan, og Miniature Synaptiske aktiviteter
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Undersøgelse af synaptiske vesikel Genbrug Brug af FM Farvestoffer Under Evoked, spontan, og Miniature Synaptiske aktiviteter

Article DOI: 10.3791/50557-v • 08:10 min • March 31st, 2014
March 31st, 2014 •
1, 1, 1, 2, 1
1Department of Molecular Physiology & Biophysics, University of Iowa Carver College of Medicine, 2Department of Biology & Biochemistry, University of Bath

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Vi beskriver brugen af ​​styryl FM farvestoffer til billede synaptiske vesikel genbrug i funktionelle nerveender. Denne protokol kan anvendes ikke blot på fremkaldte, men også spontan og miniature synaptiske aktiviteter. Protokollen udvider forskellige synaptiske begivenheder, der effektivt kan evalueres.

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Neuroscience Præsynaptiske Terminals Synaptic Blærer mikroskopi Biologisk Assay nervesystem Endocytose exocytose fluorescensimagografi FM farvestof neuron fotoblegning
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