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JoVE Journal
Bioengineering

This content is Open Access.

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
 
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Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Article DOI: 10.3791/50566-v 14:09 min December 12th, 2013
December 12th, 2013

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Summary

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Click here for the English version.

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

Tags

Localization Quantification Carbon Nanotubes Cells Multispectral Imaging Flow Cytometry Detection Distribution Innovative Method High-throughput High Resolution Imaging Statistically Relevant Image Analysis Bright-field Dark-field Fluorescent Channels Light Absorption Light Scattering Pixel-by-pixel Comparison Intrinsic Properties Interactions Between Nanomaterials
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