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Journal / Immunology and Infection / Isolation, rensning og mærkning af museknoglemarv …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation, rensning og mærkning af museknoglemarv Neutrophils for Funktionelle Studier og adoptiv overføring Experiments
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Journal (Immunology and Infection)
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Isolation, rensning og mærkning af museknoglemarv Neutrophils for Funktionelle Studier og adoptiv overføring Experiments

Article DOI: 10.3791/50586-v • 07:28 min • July 10th, 2013
July 10th, 2013 •
1, 1
1Fungal Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH

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Vi beskriver en protokol til isolering og oprensning af neutrofiler fra mus knoglemarv ved densitetsgradientcentrifugering og neutrofil mærkning hjælp CellTracker farvestoffer. Dette udgør en enkel, hurtig, reproducerbar og økonomisk metode til opnåelse af store antal neutrofiler til downstream funktionelle undersøgelser eller adoptiv overførsel og sporing eksperimenter.

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Immunologi cellebiologi infektion Infectious Diseases Molekylærbiologisk Institut Medicin Biomedical Engineering Bioengineering Neutrophils adoptiv overføring immunologi Neutrophils mus knoglemarv adoptiv overførsel densitetsgradient mærkning CellTracker celle isolation flowcytometri dyremodel
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