Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Molecular Probes geneticamente codificati a…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Molecular Probes geneticamente codificati a studiare G recettori accoppiati alle proteine
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
Journal (Biology)
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Journal (Biology)
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
 
Click here for the English version

Molecular Probes geneticamente codificati a studiare G recettori accoppiati alle proteine

Article DOI: 10.3791/50588-v • 16:16 min • September 13th, 2013
September 13th, 2013 •
1, 1, 1, 1, 1
1Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Abbiamo geneticamente codificare l'amminoacido non naturale, p-azido-L-fenilalanina in varie posizioni mirate a GPCR e mostra la versatilità del gruppo azido in diverse applicazioni. Questi includono una tecnologia photocrosslinking mirato a identificare i residui nella tasca-ligando di un GPCR, e site-specific modifica bioorthogonal di GPCR con un tag peptide-epitopo o sonda fluorescente.

Tags

Genetica Recettori G-protein-coupled Proteina Ingegneria trasduzione del segnale Biochimica acido Innaturale amino mutagenesi sito-specifica G recettore accoppiato a proteina photocrosslinking mirata etichettatura bioorthogonal mirati epitopo etichettatura
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter