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Journal / Bioengineering / CometChip: Eine Hochdurchsatz-96-Well-Plattform…
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Bioengineering

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CometChip: Eine Hochdurchsatz-96-Well-Plattform für Mess DNA-Schäden in menschlichen Zellen Microarray

Article DOI: 10.3791/50607-v • 10:59 min • October 18th, 2014
October 18th, 2014 •
*1, *2, 3, 1, 1, 2, 2, 1
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
* These authors contributed equally

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Wir beschreiben hier eine Plattform, die Comet-Assay Nachweis von DNA-Schäden mit beispielloser Durchsatz ermöglicht. Die Gerätemuster Säugerzellen in einem Microarray und ermöglicht die parallele Verarbeitung von 96 Proben. Der Ansatz erleichtert Analyse der Ausgangsniveau von DNA-Schäden, die Exposition induzierten DNA-Schäden und DNA-Reparatur-Kinetik.

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Bioengineering Comet-Assay Elektrophorese Microarray DNA-Schäden DNA-Reparatur
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