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Journal / Bioengineering / CometChip: En hög kapacitet 96-Well-plattformen…
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Bioengineering

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CometChip: En hög kapacitet 96-Well-plattformen för att mäta DNA-skador i Microarrayed humana celler

Article DOI: 10.3791/50607-v • 10:59 min • October 18th, 2014
October 18th, 2014 •
*1, *2, 3, 1, 1, 2, 2, 1
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
* These authors contributed equally

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Vi beskriver här en plattform som tillåter comet assay detektion av DNA-skador med oöverträffad genomströmning. Enheten mönster däggdjursceller i en microarray och möjliggör parallell bearbetning av 96 prover. Ansatsen underlättar analysen av basnivån DNA skada, exponering-inducerad DNA-skador och DNA-reparation kinetik.

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Bioteknik comet assay elektrofores microarray DNA-skador DNA-reparation
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