A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:58Isolate Mouse Colon Tissue
- 02:07Obtain Human Colon Tissue and Denuding Epithelial Cells
- 04:04Myofibroblast Isolation and Culture
- 05:27Representative Primary Myofibroblast Culture Results
- 06:37Conclusion
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
