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Journal / Immunology and Infection / Establishment of an In vitro System to Study…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
Journal (Immunology and Infection)
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Journal (Immunology and Infection)
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
 
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Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Article DOI: 10.3791/50625-v • 13:48 min • December 10th, 2013
December 10th, 2013 •
1, 1, *1, *1,2, 1, 1
1Laboratory of Fungal Pathogenesis, Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India, 2Current location: VIB Department for Molecular Biomedical Research, UGent, Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium
* These authors contributed equally

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The current article outlines a protocol to establish an in vitro cell culture model system to study the interaction of a facultative intracellular human fungal pathogen Candida glabrata with human macrophages which will be a useful tool to advance our knowledge of fungal virulence mechanisms.

Tags

In Vitro System Candida Glabrata THP-1 Macrophages Cell Culture Model Microbial Pathogen Infection Virulence Strategies Opportunistic Yeast Pathogen Host Phagocytic Cells Mutant Screens Viability Analysis Immune Cell Circuitry Fungal Pathogen Evasion
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