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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa…
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Chemistry
Chemistry
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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
September 9th, 2013
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Chapters
Summary
Accurate mass measurement represents an important step during the investigation of proteins. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) can be used for such determination and its key advantage is the tolerance to salts, detergents and contaminants. Here we illustrate an accessible approach for the analysis of proteins larger than 100 kDa by MALDI-MS.
Tags
Matrix-assisted Laser Desorption/Ionization Time Of Flight (MALDI-TOF) Mass Spectrometric Analysis Intact Proteins 100 KDa Accurate Mass Determination Protein Primary Sequence Mutations Post-translational Modifications Protein Degradation Sample Homogeneity Isotope Incorporation Protein Analysis Electrospray Ionization (ESI) Denatured Proteins Composition Of Sample Buffer Salts Detergents Contaminants MALDI MS Salt Tolerance Data Acquisition Interpretation Heterogeneous Proteins High Charge State Distributions ESI SpectraRelated Videos
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