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Immunology and Infection

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JoVE Journal Immunology and Infection
Forward Genetiske tilgange i
Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model
Journal (Immunology and Infection)
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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
Journal (Immunology and Infection)
High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
 
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Forward Genetiske tilgange i

Article DOI: 10.3791/50636-v • 09:03 min • October 23rd, 2013
October 23rd, 2013 •
1, 1
1Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis, Duke University Medical Center

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Vi beskriver en metode til at udføre genetisk analyse i Chlamydia baseret på kemisk mutagenese og hele genomet sekventering. Desuden er et system til DNA-udveksling inden inficerede celler beskrevet, som kan anvendes til genetisk kortlægning. Denne metode kan være bredt anvendelig til mikrobielle systemer mangler transformation systemer og molekylærgenetiske værktøjer.

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Immunologi genetik kemisk mutagenese hele genomet sekventering
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