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Dyrkning af human Nasal epithelialceller på Air Liquid grænseflade
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Dyrkning af human Nasal epithelialceller på Air Liquid grænseflade

Article DOI: 10.3791/50646-v • 10:38 min • October 8th, 2013
October 8th, 2013 •
1, 1, 1,2, 1,3, 1,2,4
1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill

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Nasale epitelceller, opnået gennem overfladisk skrabe biopsi af frivillige, der er udvidet og overført til vævsdyrkningssystemer skær. Efter at have nået konfluens, dyrkes celler på luft væske-grænsefladen, hvilket gav kulturer af cilierede og ikke-cilierede celler. Differentierede nasale epitel cellekulturer give levedygtige eksperimentelle modeller til at studere det respiratoriske slimhinde.

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Cellebiologi epitel cellekulturmodeller cilierede luftforurening vævsmodeller næseepitelet
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