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Die Kultivierung der menschlichen Nasen Epithelzellen an der Air flüssig-Grenzfläche
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Die Kultivierung der menschlichen Nasen Epithelzellen an der Air flüssig-Grenzfläche

Article DOI: 10.3791/50646-v • 10:38 min • October 8th, 2013
October 8th, 2013 •
1, 1, 1,2, 1,3, 1,2,4
1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill

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Nasen Epithelzellen, durch oberflächliche Schürf Biopsie der menschlichen Freiwilligen erhalten, werden erweitert und auf Gewebekultureinsätze übertragen. Nach Erreichen der Konfluenz werden die Zellen an der Luft Flüssigkeit-Grenzfläche angebaut, was Kulturen der Wimper und nicht-Geißelzellen. Differenzierte Nasen epithelialen Zellkulturen bieten tragfähige experimentelle Modelle für die Untersuchung der Schleimhaut der Atemwege.

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Cellular Biology Ausgabe 80 Epithel Zellkulturmodelle Flimmerepithel Luftverschmutzung Co-Kultur-Modelle Nasenepithel
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