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Journal / Biology / Odling av Human Nasal epitelceller på Air Liquid…
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Odling av Human Nasal epitelceller på Air Liquid Interface
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Odling av Human Nasal epitelceller på Air Liquid Interface

Article DOI: 10.3791/50646-v • 10:38 min • October 8th, 2013
October 8th, 2013 •
1, 1, 1,2, 1,3, 1,2,4
1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill

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Nasala epitelceller, som erhållits genom ytliga skrap biopsi av frivilliga försökspersoner, expanderas och överförs till vävnadskulturinsatser. Vid ankomsten till konfluens odlas celler vid luft flytande gränssnitt, vilket ger kulturer av cilierade och icke-cilierade celler. Differentierade nasala epitelceller cellkulturer ger livskraftiga experimentella modeller för att studera luftvägarna slemhinna.

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Cellular Biology epitel Cellodlings modeller cilierade luftföroreningar co-kultur-modeller näsepitel
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