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Rapid-Protokoll für die Herstellung von Elektrokompetente
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Rapid-Protokoll für die Herstellung von Elektrokompetente

Article DOI: 10.3791/50684-v • 07:11 min • October 8th, 2013
October 8th, 2013 •
1, 2, 2, 1,3
1Department of Biological Sciences, University of Texas Brownsville, 2Department of Medical Microbiology and Immunology, University of Alberta, 3Department of Biomedical Sciences, University of Texas Brownsville

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Elektroporation ist ein häufig verwendetes Verfahren zur Einführung von DNA in Bakterien in einem als Transformation bekannt. Traditionellen Protokolle zur Herstellung von elektrokompetenten Zellen sind zeitaufwendig und arbeitsintensiv. Dieser Artikel beschreibt eine alternative, schnelle und effiziente Methode zur Herstellung von elektrokompetenten Zellen derzeit von einigen Labors eingesetzt.

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Bioengineering Cell Engineering Gramnegative Bakterien Enterobacteriaceae Escherichia, Vibrionaceae Vibrio, Bakterien, Electrocompetence Transformationsprotokoll Elektroporation
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