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Rapid Protokoll for Utarbeidelse av Electrocompetent
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Rapid Protokoll for Utarbeidelse av Electrocompetent

Article DOI: 10.3791/50684-v • 07:11 min • October 8th, 2013
October 8th, 2013 •
1, 2, 2, 1,3
1Department of Biological Sciences, University of Texas Brownsville, 2Department of Medical Microbiology and Immunology, University of Alberta, 3Department of Biomedical Sciences, University of Texas Brownsville

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Electroporation er en vanlig anvendt metode for å innføre DNA inn i bakterier i en prosess kjent som transformasjon. Tradisjonelle protokoller for fremstilling av electrocompetent celler er tidkrevende og arbeidskrevende. Denne artikkelen beskriver en alternativ, rask og effektiv metode for fremstilling av electrocompetent celler som for tiden anvendes av noen laboratorier.

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Bioteknologi Cell Engineering Gram-negative bakterier Enterobacteriaceae Escherichia, Vibrionaceae Vibrio, Bakterier, Electrocompetence transformasjon protokollen elektroporering
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