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Storskala Gene Knockdown i

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Storskala Gene Knockdown i

Article DOI: 10.3791/50693 • 18:38 min

September 25th, 2013 •

Kathryn N. Maher¹, Mary Catanese², Daniel L. Chase³

¹Molecular and Cellular Biology Program, University of Massachusetts, Amherst, ²Neuroscience and Behavior Program, University of Massachusetts, Amherst, ³Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

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September 25th, 2013

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Mens dsrna fôring i C. elegans er et kraftig verktøy for å vurdere gen-funksjon, gjeldende protokoller for storskala fôrings skjermene resultere i variable knockdown effektivitet. Vi beskriver en forbedret protokoll for å utføre storskala RNAi fôring skjermer som resulterer i svært effektiv og reproduserbar knockdown av genuttrykk.

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