Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Grootschalige Gene Knockdown in
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Grootschalige Gene Knockdown in
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Journal (Biology)
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
Journal (Biology)
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
 
Click here for the English version

Grootschalige Gene Knockdown in

Article DOI: 10.3791/50693-v • 18:38 min • September 25th, 2013
September 25th, 2013 •
1, 2, 3
1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Terwijl dsRNA voeden in C. elegans is een krachtig hulpmiddel om gen-functie, de huidige protocollen voor grootschalige voeden schermen resulteren in variabele knockdown efficiëntie te beoordelen. We beschrijven een verbeterde protocol voor het uitvoeren van grootschalige RNAi voeden schermen die resulteert in een hoogst doeltreffend en reproduceerbare neerhalen van genexpressie.

Tags

Developmental Biology Caenorhabditis elegans (C. elegans) Gene Knockdown Technieken, DsRNA interferentie gen knock-down grootschalige voeden scherm
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter